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Chem Impex International
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Carl Roth GmbH
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Merck KGaA
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Sinopharm ltd
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Merck KGaA
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Beijing Solarbio Science
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Merck KGaA
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Scientific Safety Solvents Inc
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ECHO Chemical Co Ltd
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Carl Roth GmbH
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Bartelt GmbH
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Merck KGaA
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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Fast, Precise, and Reliable Multiplex Detection of Potato Viruses by Loop-Mediated Isothermal Amplification
doi: 10.3390/ijms21228741
Figure Lengend Snippet: Initial testing of the tobacco rattle virus (TRV)-LAMP method. The NEB WarmStart qRT-LAMP method was tested with HPLC-purified primer mix 1 (PM1). Template = RNA purified from TRV-infected (positive, green) and uninfected (negative, blue) tuber tissues. The total reaction volume was 10 µL (including 0.5 µL template, 0.5 µL primer mix and 0.1 µL 50× LAMP dye). Positive control (PC, orange) = German Collection of Microorganisms and Cell Cultures (DSMZ) virus isolate TRV PV-0352. Negative non-template control (NC, gray) = milliQ water. For visual clarity, the amplification plots ( a ) show the increase in fluorescence with the number of cycles, and the bar chart ( b ) shows the overall Ct values. RT-LAMP tests were monitored for 1 h (1 min/cycle).
Article Snippet: Filtered supernatant was mixed with an equal volume of
Techniques: Virus, Purification, Infection, Positive Control, Control, Amplification, Fluorescence
Journal: International Journal of Molecular Sciences
Article Title: Fast, Precise, and Reliable Multiplex Detection of Potato Viruses by Loop-Mediated Isothermal Amplification
doi: 10.3390/ijms21228741
Figure Lengend Snippet: TRV LAMP primer mix comparison. NEB WarmStart qRT-LAMP tests with ( a ) HPLC-purified primer mix 1 (TRV-PM1) or ( b ) TRV-PM2 with new forward internal primer (FIP) and backward internal primer (BIP). Template = RNA purified from TRV-infected (positive, green) and uninfected (negative, blue) tuber tissues. We used 1 µL (1:10 dilution) of template together with positive and negative controls in a total volume of 10 µL. Positive control (PC, orange) = DSMZ virus isolate TRV PV-0352. Negative non-template control (NC, gray) = milliQ water. RT-LAMP tests were monitored for 1 h (1 min/cycle).
Article Snippet: Filtered supernatant was mixed with an equal volume of
Techniques: Comparison, Purification, Infection, Positive Control, Virus, Control
Journal: International Journal of Molecular Sciences
Article Title: Fast, Precise, and Reliable Multiplex Detection of Potato Viruses by Loop-Mediated Isothermal Amplification
doi: 10.3390/ijms21228741
Figure Lengend Snippet: Optimization of the potato virus X (PVX) RT-LAMP protocol. We used qRT-LAMP to amplify PVX-positive controls (PV-0014, PV-0020, PV-0847, and PV-1101), negative control (NC-0017), and negative potato tuber RNA (N11 and N12) in two dilutions (1/200 and 1/1600). We used PVX primer mix 4 (PVX-PM4) standard desalted, with HPLC-purified FIP/BIP, or with and additional loop primer. We added 450 mM betaine or 7.5% ( v / v ) DMSO to the reaction mix. 1 For the Ct value, one cycle = 1 min.
Article Snippet: Filtered supernatant was mixed with an equal volume of
Techniques: Virus, Negative Control